Agarose gel
Bioluminescence
Camera lens focal lengths
CCD camera
Chemiluminescence
Cloning
Colony counting
Colorimetric blots
DGGE
DIGE 2D gels
DNA
F-Stop
Film
Fluorescence
Fluorophore
Gel documentation
Gel electrophoresis
Gel imaging
GFP plant imaging
In-cell western
In-Gel western
In-vivo imaging
Infra-red imaging
Lens
Membrane
Molecular weight ladder
Multiplexed western blot
Nitrocellulose membranes
PCR
Polyacrylamide gel
Proteins
PVDF membranes
Safe dye imaging
Signal to noise ratio
Stain Free Gels
TGX Stain-free™ gels
Thin Layer chromatography (TLC)
UV shadowing
Western blotting
Glossary definition of
Definition of Membrane
There are two main types of membranes PVDF (polyvinylidene difluoride) and nitrocellulose, both available in different pore sizes and dimensions depending on your application needs.
PVDF membranes are highly hydrophobic and must be pre-wetted with methanol. This type of membrane has a high binding affinity for proteins and nucleic acids and is often used for the following applications - western, southern, northern and dot blots.
Nitrocellulose membranes are popular in protein blotting due to its high-binding affinity and compatibility with a variety of detection methods including chemiluminescence, chromogenic and fluorescence.