Rapid set-up and image capture of 1D, 2D and multiplex gels

Syngene's revolutionary new GeneSys image acquisition software has been developed for use with G:BOX and Dyversity systems. GeneSys ensures scientists can quickly capture excellent images of even complex multiplex gels.

The innovative GeneSys software features large touch-screen buttons which guide users effortlessly through set-up and image capture. Each screen prompts researchers to select, for example, the type of gel or blot they are using and what it is stained with.

Features

The biggest difference between a Syngene system and any other is in the way the system is controlled. At the heart of all Syngene systems is GeneSys which is an application driven control software. With G:BOX you can use GeneSys in either Automatic or Manual mode.

It is assumed that the user will know exactly what their application is and how they have prepared their gel or blot. In Auto Mode they simply enter this information into GeneSys (or recall it from a saved configuration) and the system takes over the rest. Behind GeneSys is an extensive database which contains data relating to a very wide range of applications, eg, fluorescence, chemiluminescene and chemifluorescence. Once the G:BOX has been told what sample to expect then GeneSys decides what hardware configuration is best and sets the system ready for image capture. Things like camera control, exposure time, sensitivity setting, lighting requirements, lens control, filter selection – all of this is taken care of by GeneSys. The user just has to click the ‘capture’ button and wait for the perfect image to appear.

From the opening screen the user can select the mode of operation – Auto or Manual. It is also possible to access the image browser and enter the edit screen to work on previously stored images.  Previously stored configurations are shown on the left as small ‘notebooks’.

In Auto Mode the user simply selects the ‘sample format’, the ‘sample type’ and the ‘matrix type’. The software has been designed for use with a touch screen.

Once the application type has been selected, GeneSys then offers the user a range of dyes from its database. The user then simply selects the dye. It is also possible to undertake multiplex applications in which case the user would select each dye from the available list. The database behind GeneSys contains over 210 different dyes and stains and is updated on a regular basis as more options come onto the market.

Once the dye has been selected, the system will automatically configure itself to capture the best possible image of the sample. The lighting, filter and camera exposure settings are all automatically adjusted for the sample type. The captured image is displayed in the main screen with small thumbnail images of the current and previous images (either opened or captured) shown in an image ‘pool’. Note the icons at the foot of the screen which enable previous pages to be selected.

The fully automatic functions of GeneSys allow even the most difficult or complicated gels and blots to be imaged. For example, with Chemiluminescence the system uses its intelligence to know the output profile of most of the major substrates. It can then decide the exposure setting best suited to a particular substrate. Then during the image capture process it continually monitors the output from the blot until the best image is produced.

Another example of the power of GeneSys is when working with multiplex blots. Once the application information is selected by the user GeneSys will make separate exposures using the correct filter, lighting and camera setting for each dye. The final multiplex image will be displayed.

On the image edit page the user can enhance the image, add annotation, save or print.